Resveratrol (Resv), a polyphenolic compound have the anti-inflammatory and antioxidant effects. Tristetraprolin (TTP) destabilizes mRNAs of pro-inflammatory cytokines. However, it was not clear how Resv exerts anti-inflammatory effects on hepatocytes. In this study, we used human hepatoma cell line (HepG2 cells) and analyzed samples using qRT-PCR. Resv increases TTP expression in hepatocytes. Resv attenuates LPS-induced inflammation through TTP expression in hepatocytes. In TTP deficient hepatocytes, LPS failed to attenuate TNFa, iNOS, and IL-6 mRNA expression whereas TTP level was still decreased. Our results suggest that Resv may attenuate inflammation in hepatocytes through TTP induction at least in part. This novel Resv-TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases in liver.

Di (n-butyl) phthalate (DBP), a potential endocrine disruptor, adversely affects male reproductive function via activation of oxidative stress. This study was conducted to investigate the protective effects of vitamin E (Vit E) and selenium (Se) on DBP-induced testicular damage. Prepubertal rats were given 500 mg DBP/kg alone and in combination with Vit E (250 mg/kg) & Se (1 mg/kg) by oral gavage for 7 days and sacrificed at day 1 (D1), D30 and D45 after administration. Testicular section of DBP-treated rats showed markedly distorted seminiferous tubules, no spermatids and a reduction in the thickness of their epithelial lining, compared to the control. In contrast, co-administration of Vit E & Se protected the seminiferous tubules and regained its normal architecture to the control level. To clarify whether spermatogenic cells differentiate into mature spermatids in the treated testes at the end of first wave of spermatogenesis, immunostaining for Hsc 70t, a specific marker for spermatids, was carried out. As a result, the increase in maturation of spermatids in Vit E & Se+DBP-treated testes, compared to the DBP-treated, was demonstrated. For example, the most advanced spermatids in the tubules from rats in the DBP-treated groups were steps 8-9 at D45 of recovery, while those of the DBP+Vit E & Se-treated were steps 14-19 that were more or less similar to the control group. These results show for the first time that prepubertal administration of Vit E & Se have protective effects on DBP-induced testicular damage and restoration of normal spermatogenesis.

Endothelial dysfunction is one of the earliest pathological features in atherosclerosis. The current study designed to analyse the telomere length of human coronary artery derived endothelial cells to identify the relationship between telomere shortening and coronary artery disease (CAD)s. Coronary artery endothelial cells obtained from 30 patients with CAD and cell line were analysed by real time PCR method. Relative telomere and single-copy 36B4 gene (T/S) ratio which corresponds to the telomere length in coronary artery was significantly smaller in coronary artery disease patients than compared with control cell line. Flow cytometry analysis resulted endothelial cell count ranged from 0.55 x 106 to 0.96 x 106. Findings of this study suggest that telomere shortening in coronary artery endothelial cells play important role in the coronary artery disease.

Ocimum genus most commonly termed as Tulsi, an aromatic plants belongs to family Lamiaceae. In addition to anti bacterial activity, anti-oxidant, anti diabetic, cardio protective, neuroprotective, radio-protective, anti-cancer, and free radical scavenging properties, quite a few studies have reported that this traditionally grown medicinal herb may become a potential therapy for diabetic kidney disease (DKD). This paper will significantly review Tulsi plants role in treatment of DKD to provide reference for future research.

Two minnows, indigenous olive barb, Puntius sarana and exotic silver barb, Barbodes gonionotus are important fish species in Bangladesh. Therefore, it is essential to identify diversed population of these fish species for selective breeding programme. Sixty olive barb fish were collected from three different natural stocks (Mymensingh, Madaripur and Sylhet) and 20 silver barbs from hatchery stock of Jashore in Bangladesh. Out of 40 decamer primers tested, 5 primers were selected for the Polymerase Chain Reaction (PCR) based RAPD (Randomly Amplified Polymorphic DNA) analysis. Upon agarose gel electrophoresis, RAPD bands were scored as separately on the basis of their presence or absence for each sample and primer. A total of 43 polymorphic bands and highest proportion of polymorphic bands (62.79%) were found in the Madaripur populations. The gene diversity (0.2132±0.2067) and Shannon’s Information index (0.3161±0.2950) within populations were highest in Jashore stock. Among olive barb stocks, these values were higher in Sylhet stock. Besides, four populations segregated in two main clusters based on the Nei’s genetic distance. Indicating the segregation of two different species of minnows, the silver barb stock made one separate cluster while other three stocks of olive barb remained in another cluster. The present study exposed a distinct pattern of genetic variation and phylogenetic relatedness that would be helpful in selecting broodfish for genetic improvement as well as in conservation of these fish species.